Critical Role of Gut Microbiota in the In Vivo Biotransformation of Nobiletin: Insights Into Metabolic Pathways and Bioactive Metabolite Formation

Results: We initially evaluated the basal mRNA levels of Class I facilitative Gluts: Glut1, Glut2, Glut3 and Glut4 in the mouse RAW264.7 macrophages.Relative to Glut1 mRNA, Glut3 was more abundant than Glut1 but Glut4 was much less and Glut2 was undetectable in the macrophages.qPCR results showed that both gossypol and LPS stimulated Glut gene expression but gossypol was a stronger stimulator in regulating Glut gene expression in the mouse macrophages.Conclusions: This study suggest the potential value of gossypol for regulating macrophage immunity.