Resistant Starch Supplementation Enhances Gut Barrier Integrity in Estrogen Deficient Mice by Modulating the Gut Microbiota

1 phylum, 2 families, and 4 genera were lower in DHA piglets compared to control.rs-fMRI results were consistent with our previous results, with higher cerebellar and visual network activities and lower sensorimotor network activity in DHA piglets compared to control.Notably, the visual network was positively correlated with Pielou's evenness and Shannon diversity index, while salience network was negatively correlated with Faith's phylogenetic diversity and the number of observed features (p< 0.05).Significant correlations were observed between various networks and the relative abundance of fecal microbes, such as the association of the Firmicutes to Bacteroidetes ratio with default mode and salience networks (p< 0.05).Conclusions: Maternal intake of DHA altered the richness, diversity, and relative abundance of piglet microbiota.The functional networks were correlated with microbial composition, suggesting that the effect of maternal DHA supplementation on functional connectivity of piglet brains may be partly explained by the gut-brain axis.