Fetal Liver Weight Is Negatively Associated With Maternal Plasma Lipid Metabolites in a Mouse Model of Prenatal Alcohol Exposure and Choline Supplementation

DNA was extracted from stool (50-75 mg) using Qiagen QIAmp Fast Stool DNA Mini Kits and libraries were paired-end sequenced (2x250bp) using an Illumina MiSeq.Raw sequencing files were processed into amplicon sequence variant (ASV) counts using QIIME2.Results: A total of 87 and 126 women in the SOC and PA groups, respectively, had stool samples available for 16S rRNA analysis.Women in the PA group increased their GW12 submaximal VO2 (workload corresponding to 15 on Borg's scale) 9.9% by GW24, compared to a 5.5% decrease in the SOC group between GW12 and GW24 (P < 0.01).Alpha diversity estimates, total ASVs, Shannon, and Simpson indices, showed no statistical differences between PA and SOC groups across all gestational periods.Similarly, no effect of PA was observed across any gestational period for the beta-diversity estimate, Bray-Curtis Dissimilarities.Using DESeq2 and ALDEx2 workflows, only 2 ASVs were statistically different after FDR correction at GW36: An ASV classified to the Prevotella genus was lower in the PA group while an ASV classified to the Akkermansia genus was greater in the PA group relative to SOC.Conclusions: A structured and supervised submaximal physical activity intervention improved the aerobic capacity in pregnant women with obesity, but had minimal effect on the composition of the fecal microbiota.