Suppression of Renal Pro-inflammatory Cytokines by Fructooligosaccharides Is Associated With Renal Vitamin D Signaling in High-Fat Diet-Induced Obese C57BL/6 Mice

or a zinc supplemented (ZS, 300 mg Zn/kg diet) diet for 6 weeks (n8-10 mice/treatment group).Tibias were collected and evaluated using dual energy x-ray absorptiometry (whole tibia) and microcomputed tomography (proximal tibia metaphysis).Results: In both experiments, cancellous bone volume fraction, connectivity density, and trabecular number were lower, while trabecular thickness and spacing were higher in old mice (ZA young vs ZA old).Neither 6 weeks of marginal zinc deficiency nor 6 weeks of zinc supplementation had any effect on the cancellous bone parameters measured in either age group (same age ZA vs MZD or same age ZA vs ZS).In addition, neither age nor zinc status affected total tibia bone mineral density.Conclusions: In summary, aging had profound effects on cancellous bone microarchitecture in proximal tibia in male mice, with cancellous bone volume decreasing due to a reduction in trabecular number.In contrast, short-term exposure to nutritionally relevant zinc supplementation, or marginal zinc deficiency, had little impact on bone microarchitecture in young or old mice.