Chemical proteomics enhances the understanding of 2AA stress in <i>Salmonella enterica</i>

Loss of RidA homologs results in 2-aminoacrylate stress in <i>Salmonella</i> and other bacteria. The stress is derived from the reaction of 2AA with a pyridoxal phosphate cofactor in metabolic enzymes, which inactivates the respective enzymes. This study uses a chemical proteomic method and, with an initial test case, explores the damage that is generated by 2AA on a global proteomic scale. This work provides a basis for probing the extent of 2AA stress in different organisms and for identifying the enzymes targeted by 2AA.