Comparative Study of Hydroxyproline Concentration in the Periprosthetic Capsule after Subcutaneous Implantation of Hemostatic Sponges in a Chronic in vivo Experiment

The aim of the study was to evaluate the dynamics of hydroxyproline concentration after subcutaneous implantation of local hemostatic agents in an in vivo experiment. The experiment was carried out on 210 male Wistar rats divided into seven groups (30 animals in each). Under inhalation anesthesia, an incision was made in the skin and subcutaneous fat of laboratory animals along the scapular line, then a tool was inserted into the wound, forming a pocket. Samples were implanted into the resulting depression according to the division into groups. To assess the dynamics of hydroxyproline concentrations, animals were taken out on the 7th, 14th and 28th days. After CO2-induced euthanasia, the area of the periprosthetic capsule was isolated. After sample preparation of the biomaterial, the concentration of hydroxyproline was determined by chromatographic method. The statistical significance of differences was determined by calculating the Kruskal-Wallis test. In all experimental groups, a direct correlation was revealed between the concentration of 4-hydroxy-L-proline and the timing of elimination. On the 28th day, the highest concentration of hydroxyproline was detected in experimental groups No. 5 (0.0285), 7 (0.0160), 3 (0.0110), and the lowest in groups No. 4 (0.0058), 6 (0.0058), 2 (0.0078), 1 (0.0082). These indicators can be associated with the high collagen content in implants, which provokes a more pronounced local reaction of the macroorganism. Subcutaneous implantation of combined hemostatic sponges based on NaCMC and deep-sea squid collagen statistically significantly (p ≤ 0.05) provoke a less pronounced tissue reaction compared with control and comparison groups.