EVALUATION OF ANTI- THROMBOTIC ACTIVITY OF CHRYSANTHEMUM INDICUM FLOWERS- AN IN VITRO STUDY

Cardiovascular diseases involving deep vein thrombosis, stroke, hypertension and heart attacks are the main causes of morbidity and mortality in world.Synthetic drugs used in such disorders leads to serious adverse effects.The discovery of the natural products in the treatment of these diseases reduce the side effects.Inhibiting the aggregation off platelets prevents the formation of thrombus.This is the most widely used mechanism to determine the potential efficacy of anti-thrombotic agents.So the aim of present study was to evaluate the in vitro antithrombotic activity of hydroalcoholic extract of Chrysanthemum indicum flowers.In this study was evaluated the anti-thrombotic activity of hydroalcoholic flower extract of Chrysanthemum indicum by using clotting time assay and Cacl2 induced clotting time assay.The inhibitory activity of Chrysanthemum indicum flower extract was comparable with the standard drug.The percentage inhibition of Chrysanthemum indicum flower extract was attributed due to the presence of flavonoids, phenols, carotenoids.IC50 values of Chrysanthemum indicum flowerextract by using clotting time assay and Cacl2 induced assay were found to be 41.96 and 49.75 g/ml respectively.Whereas the IC50 value of standard was found to be 24.36 g/ml.