ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METOPROLOL TARTRATE, AMLODIPINE BESYLATE AND CHLORTHALIDONE IN SYNTHETIC MIXTURE

ABSRACTSimple, specific, accurate, precise and reproducible and robust method have been developed and validated for the Simultaneous Estimation of Metoprolol tartrate, Amlodipine besylate and Chlorthalidone in synthetic mixture.The Reverse Phase High Performance Liquid Chromatography, the chromatographic system was equipped with Cosmosil C18 (250 mm 4.6 mm, 5 m) and UV detector set at 222 nm, in conjunction with a mobile phase of Phosphate Buffer pH 2.5: ACN: Methanol in the ration of (50:30:20%v/v), at a flow rate of 1.5 mL/min.Retention time was noted to be 1.44, 1.33 and 1.13 for Metoprolol Tartrate, Amlodipine Besylate and Chlorthalidone respectively.The various analytical parameters, including specificity, linearity, LOD, LOQ, precision, accuracy, and robustness, were determined ICH guidelines.The method was linear over the range of 245.53-736.60 g/ml, 25.06-75.18g/ml and 24.81-74.44g/ml for Metoprolol Tartrate, Amlodipine Besylate and Chlorthalidone respectively.The % recoveries for Metoprolol Tartrate was noted to be 101.7%-100.5%,98.4% -101.3%for Amlodipine Besylate and 100.7% -99.8% for Chlorthalidone.The developed RP-HPLC method was found to be specific, accurate, precise, robust for simultaneous estimation of Metoprolol Tartrate, Amlodipine Besylate and Chlorthalidone in synthetic mixture and stable for routine