Comparison of turnaround time (TAT) performance between Enzyme-Linked Immunosorbent Assay (ELISA) vs. Automated Chemiluminescent Immunoassay (CLIA) in measuring diabetes autoantibodies

Autoanbodies to glutamate decarboxylase (GAD), islet angen-2 (IA2) and islet cell angen (ICA) are characterisc markers for type 1 diabetes among paediatric populaon and adults with latent autoimmune diabetes.We aim to compare the analysis performance of the enzymelinked immunosorbent assay (ELISA) and the chemiluminescence immunoassay (CLIA) methods in the detecon of diabetes autoanbodies.A cross-seconal study was conducted on 1,425 serum samples sent for diabetes autoanbodies measurement at Instute for Medical Research, Kuala Lumpur, Malaysia from April 2021 to July 2022.A total of 695 samples were measured using the Medizym ELISA kit 96 wells and 730 samples were measured using Maglumi CLIA kit.Both methods were able to produce mely results at more than 90% of the allowable laboratory turnaround me (LTAT).Cohen's kappa showed good and sasfactory agreement between the two methods: 0.734 (GAD), 0.413 (ICA), 0.514 (IA2).Addionally, CLIA method was significantly less me-consuming and less labour-intensive.CLIA method has a significant advantage over ELISA in offering beter laboratory workflow with shorter me tesng and TAT in laboratory pracce.