Development of a low-cost equipment alternative to the Kjeldahl distiller for the determination of total volatile basic nitrogen
The determination of the total volatile basic nitrogen content in fish is a method of assessing food quality that is typically carried out using the Kjeldahl distiller. The objective of this project was to develop an alternative equipment configuration to replace the Kjeldahl distillation apparatus. The results obtained are promising, as the ammonia extraction achieved 98% efficiency and the TVB-N content and relative standard deviation were 31.0 mg N per 100 g and 11.6%, respectively. The same samples studied using the traditional method showed TVB-N content and relative standard deviation of 32.5 mg N per 100 g and 12.4%, respectively. It can be concluded that the newly developed low-cost equipment can be useful for determining TVB-N without compromising the results of routine analyses previously obtained exclusively through the Kjeldahl method for evaluating fish quality. In addition, the alternative equipment is safer and allows for a higher analytical frequency compared to the Kjeldahl distiller.
Authors
- Luciano Pereira Rodrigues (ORCID: https://orcid.org/0000-0002-9488-5000)
- Flaviano Oliveira Silvério (ORCID: https://orcid.org/0000-0002-5753-8739)
- Amanda Maria Leal Pimenta (ORCID: https://orcid.org/0000-0001-5827-8193)
- André Fernando de Oliveira (ORCID: https://orcid.org/0000-0003-4158-6098)
- Fernanda Fernandes Heleno (ORCID: https://orcid.org/0000-0002-0423-5662)
- Antônio Augusto Neves (ORCID: https://orcid.org/0000-0002-2152-6736)
- José Humberto de Queiróz (ORCID: https://orcid.org/0000-0001-8196-4255)
- Flávio Pinto Monção (ORCID: https://orcid.org/0000-0002-9821-0107)
- Luís Felipe Rodrigues Costa
- Nelson de Abreu Delvaux Júnior (ORCID: https://orcid.org/0000-0001-7150-1521)
- Fernanda Menezes Maia
Institutions
- Universidade Federal de Minas Gerais (BR)
- Universidade Federal de Viçosa (BR)
- Universidade Federal dos Vales do Jequitinhonha e Mucuri (BR)
- Universidade Estadual de Montes Claros (BR)
- Universidade Federal de Uberlândia (BR)
Publication Details
- Journal
- Analytical Methods
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5ay00260e
- Citations
- 1
- Primary Topic
- Advanced Chemical Sensor Technologies
- Type
- article
- Field-Weighted Citation Impact
- 0.67
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais