Intestinal microbiome profile of the brown rock sea cucumber ( <i>Holothuria glaberrima</i> ) using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction
Using direct mechanical, enzymatic, and chemical extraction methods, the intestinal microbiome of the marine invertebrate <i>Holothuria glaberrima</i> was obtained. ITS and 16S rDNA regions were sequenced to enrich and investigate the prokaryotic and fungal diversity profiles from different anatomical regions within the sea cucumber's intestinal biology.
Authors
- Josué A. Rodríguez-Ramos (ORCID: https://orcid.org/0000-0002-2049-2765)
- Edwin Omar Rivera-Lopez (ORCID: https://orcid.org/0000-0001-6118-0816)
- Carlos Rı́os-Velázquez (ORCID: https://orcid.org/0000-0002-3685-0838)
- José E. García‐Arrarás (ORCID: https://orcid.org/0000-0001-9038-7330)
- Rene Nieves-Morales
- Gabriela Melendez-Martinez (ORCID: https://orcid.org/0000-0002-5524-4497)
- Jessica Alejandra Paez-Díaz (ORCID: https://orcid.org/0009-0008-6418-9232)
- Sofia Marie Rodriguez-Carrio
Institutions
- Pennsylvania State University (US)
- Pacific Northwest National Laboratory (US)
- University of Puerto Rico at Río Piedras (PR)
- University of Puerto Rico-Mayaguez (PR)
Publication Details
- Journal
- Microbiology Resource Announcements
- Published
- 2025-05-28
- DOI
- https://doi.org/10.1128/mra.00293-25
- Primary Topic
- Echinoderm biology and ecology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation of Sri Lanka