Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity

The polyene antifungal amphotericin B (AmB) can form giant helical aggregates. We show that microscopic and mesoscopic structural features of its aggregates can be revealed by Raman optical activity (ROA) and electronic circular dichroism (ECD), respectively. The ROA method, which senses molecular structure more locally, elucidates the conformation of the polyene chain of individual AmB molecules in an aggregated state. In turn, the ECD signal related to exciton coupling effects provides details about the arrangement of AmB molecules in the supramolecular structure. Thus, the use of both complementary methods will be crucial in future structural studies of chiral supramolecular systems.

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Publication Details

Journal
Analytical Chemistry
Published
2025-05-28
DOI
https://doi.org/10.1021/acs.analchem.5c01198
Primary Topic
Molecular spectroscopy and chirality
Type
article
Field-Weighted Citation Impact
0.00

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article

Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity

Masashi Unno, Marzena Mach, Małgorzata Barańśka, Grzegorz Zając et al.
Analytical Chemistry
Molecular spectroscopy and chirality
article

Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity

Masashi Unno, Marzena Mach, Małgorzata Barańśka, Grzegorz Zając, Ewa Machalska, Marco Fusè, Katarzyna Pajor, Marta Arczewska, Mariusz Gagoś, Tomotsumi Fujisawa, Yoshimitsu Onaka
article en

Abstract

The polyene antifungal amphotericin B (AmB) can form giant helical aggregates. We show that microscopic and mesoscopic structural features of its aggregates can be revealed by Raman optical activity (ROA) and electronic circular dichroism (ECD), respectively. The ROA method, which senses molecular structure more locally, elucidates the conformation of the polyene chain of individual AmB molecules in an aggregated state. In turn, the ECD signal related to exciton coupling effects provides details about the arrangement of AmB molecules in the supramolecular structure. Thus, the use of both complementary methods will be crucial in future structural studies of chiral supramolecular systems.

Analytical ChemistryVol. 97(22)
Jagiellonian University (PL), University of Life Sciences in Lublin (PL), Maria Curie-Skłodowska University (PL), Saga University (JP), Instytut Chemii i Techniki Jądrowej (PL), University of Brescia (IT)
Narodowe Centrum Nauki, Narodowym Centrum Nauki, Uniwersytet Jagielloński w Krakowie, Japan Society for the Promotion of Science
Openalex Percentile: Top 89%
Molecular spectroscopy and chirality
DOIOpen Access PDFOpenAlex
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Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity — Masashi Unno, Marzena Mach, et al. · Analytical Chemistry (2025) | TGRS Research Map | TGRS