Coordinated implementation of a conventional PCR assay to detect all Ebola and Marburg virus species in a European laboratory network
The assay enables rapid and reliable detection of filoviruses, with sequencing capabilities for identifying both known and novel variants. This assay might be used for detection during the initial phase of an emerging filovirus outbreak, before a specific assay has been developed. However, our distribution across 15 laboratories revealed variability challenges due to reagents, human performance, and sequencing capacity, emphasizing the need for more training and standardization.
Authors
- L D Presser
- Cécile Baronti (ORCID: https://orcid.org/0000-0002-2319-9484)
- Kristi Huik (ORCID: https://orcid.org/0000-0002-1569-8830)
- Styliani Pappa (ORCID: https://orcid.org/0000-0002-2249-2443)
- Richard Molenkamp (ORCID: https://orcid.org/0000-0002-9004-3850)
- Ricardo Cordeiro (ORCID: https://orcid.org/0000-0002-0437-1066)
- Jana Šmahelová (ORCID: https://orcid.org/0000-0002-9164-4279)
- Irena Tabain (ORCID: https://orcid.org/0000-0002-2518-522X)
- Anne J. Jääskeläinen (ORCID: https://orcid.org/0000-0003-3713-8110)
- Tobias Bleicker
- Anna Justyna Milewska (ORCID: https://orcid.org/0000-0001-8028-5146)
- Victor M. Corman (ORCID: https://orcid.org/0000-0002-3605-0136)
- V Pinho Dos Reis
- C. Drosten
- G Marsili
- Luisa Barzon (ORCID: https://orcid.org/0000-0003-0720-8456)
- Peter Maes (ORCID: https://orcid.org/0000-0002-7099-7681)
- K C Heimsch
- T D Best
Institutions
- Jagiellonian University (PL)
- Friedrich-Loeffler-Institut (DE)
- University of Helsinki (FI)
- University of Padua (IT)
- Inserm (FR)
- Charles University (CZ)
- Aristotle University of Thessaloniki (GR)
- Aix-Marseille Université (FR)
- Rega Institute for Medical Research (BE)
- Humboldt-Universität zu Berlin (DE)
- National Institute for Public Health and the Environment (NL)
- National Institute of Health (AM)
- Berlin Institute of Health at Charité - Universitätsmedizin Berlin (DE)
- National Institute of Health Dr. Ricardo Jorge (PT)
- Croatian Science Foundation (HR)
- Hospital District of Helsinki and Uusimaa (FI)
- Institut de Recherche pour le Développement (FR)
- University of Tartu (EE)
- Charité - Universitätsmedizin Berlin (DE)
- Erasmus University Rotterdam (NL)
Publication Details
- Journal
- Journal of Clinical Virology
- Published
- 2025-05-28
- DOI
- https://doi.org/10.1016/j.jcv.2025.105808
- Citations
- 1
- Primary Topic
- Viral Infections and Outbreaks Research
- Type
- article
- Field-Weighted Citation Impact
- 1.40
Funders
- European Commission