Genetic engineering of E. coli K-12 for heterologous carbohydrate antigen production
We have provided a convenient and reliable genomic glycoengineering method to produce efficacious, durable, and cost-effective carbohydrate antigens in non-pathogenic E. coli. Non-pathogenic E. coli glycoengineering has great potential for the highly efficient synthesis of heterologous polysaccharides and can serve as a versatile platform to produce next-generation biomedical agents, including glycoconjugate vaccines, glycoengineered minicells or outer membrane vesicles (OMVs), polysaccharide-based diagnostic reagents, and more.
Authors
- Hongyan Luo (ORCID: https://orcid.org/0000-0003-3845-2400)
- Haichun Gao (ORCID: https://orcid.org/0000-0002-2647-6563)
- Keyan Wei
- Hong‐Guang Zha (ORCID: https://orcid.org/0000-0001-5671-3999)
- F.A. Lai
- Caixia Li (ORCID: https://orcid.org/0000-0003-3757-8167)
- Pei Li (ORCID: https://orcid.org/0000-0001-7276-6337)
- Zuoyong Zhou (ORCID: https://orcid.org/0000-0002-5098-1367)
- Ziyan Jiao
Institutions
- Southwest University (CN)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-05-28
- DOI
- https://doi.org/10.1186/s12934-025-02749-2
- Citations
- 1
- Primary Topic
- Escherichia coli research studies
- Type
- article
- Field-Weighted Citation Impact
- 1.80
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Chongqing
- Centre Scientifique et Technique du Bâtiment
- Fundamental Research Funds for the Central Universities