Biodiesel production from oleaginous Saccharomyces cerevisiae by adaptive laboratory evolution method using glycerol as an alternative carbon source

In this study, oleaginous Saccharomyces cerevisiae was subjected to adaptive laboratory evolution (ALE) to improve its growth on glycerol.After 25 sequential serial passages, the ALE-evolved strain S. cerevisiae TP-25 grown on glycerol exhibited a sevenfold increase in growth compared with the parental strain.In addition, C/N molar ratio was evaluated for lipid production, and the highest lipid content was observed at a C/N molar ratio of 75.At this ratio, the TP-25 strain produced 33.27 0.55% of lipid content and 0.61 0.01 g/l of lipid production.To assess the feasibility of these lipids for biodiesel production, the lipid properties were analyzed and compared with international biodiesel standards, including ASTM D6751 and EN 14214.The findings indicated that the lipids produced by oleaginous S. cerevisiae using glycerol as the carbon source met the necessary criteria for biodiesel production.This suggested that glycerol, a byproduct of many industrial processes, could serve as a valuable carbon source for sustainable biodiesel production in oleaginous S. cerevisiae strains.