Genome Sequence Resource of <i>Flavobacterium</i> spp. Strain CGRL1 and Strain CGRL2 Isolated from Citrus Huanglongbing-Infected Trees

Flavobacterium is a gram-negative bacterium commonly found in soil and water that also forms endophytic relationships with host organisms. This study reports the draft genome and taxonomic characterization of Flavobacterium spp. strain CGRL1 and strain CGRL2 isolated from citrus huanglongbing-infected trees. According to the results of sequencing and assembly, strain CGRL1 had a total genome length of 5,642,596 bp, 5,191 coding sequences (CDSs), and a genome CG content of 34%. Strain CGRL2 had a total genome length of 5,254,724 bp, 4,778 CDSs, and a genome CG content of 34%. Phylogenetic analysis of the 16S ribosomal RNA gene revealed that both strain CGRL1 and strain CGRL2 were members of the genus Flavobacterium, clustering with Flavobacterium johnsoniae strain UW101 and Flavobacterium resistens strain BD-b365. Multilocus sequence analysis (MLSA) using six housekeeping genes and average nucleotide identity (ANI) analysis were performed. These results, based on both MLSA and ANI analyses, indicate that strain CGRL1 is closely related to F. johnsoniae, with 99.15% MLSA similarity and 96.26% ANI value. In contrast, strain CGRL2 showed only 94.04% MLSA similarity and 82.20% ANI value with F. resistens. In addition, we identified two gene clusters in strain CGRL1 and strain CGRL2 that are responsible for the biosynthesis of pigments and antibiotic compounds. Notably, in strain CGRL2, one specific gene cluster was found to be associated with biofilm formation and mitigation of host organism aging. The genome sequences of strain CGRL1 and strain CGRL2 enhance our understanding of bacterial interactions, including interbacterial competition and colonization. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .