Molecular Detection of Different Species of Cryptosporidium in Snakes from Surinam and Indonesia

In recent decades, the keeping of exotic animals has gained popularity among enthusiasts worldwide. However, alongside the development of exotic animal husbandry, issues related to health status and adequate veterinary care are coming to the forefront. The introduction of new snakes into a collection and shared enclosures should always be preceded by an assessment of their parasitic status. In our study, we present an overview of the screening for the presence of <i>Cryptosporidium</i> spp. in individuals captured in regions of Indonesia and Suriname, intended for further trade. Out of 40 tested fecal samples, the presence of cryptosporidial oocysts was confirmed in 6 samples. Detection was performed by molecular methods, namely Nested PCR targeting the <i>GP60</i> gene region (60 kDa glycoprotein). By sequencing, we confirmed the presence of <i>C. parvum</i> in <i>Oligodon octolineatus</i> (<i>n</i> = 1) and <i>Trimeresurus insularis</i> (<i>n</i> = 1), <i>C. tyzzeri</i> in <i>Corallus</i> spp. (<i>n</i> = 2), and <i>C. hominis</i> in <i>Boiga dendrophila</i> spp. <i>gemmicincta</i> (<i>n</i> = 2), which is the very first time that this species has been detected in snakes in captivity. Although the presence of <i>Cryptosporidium</i> species, typical for snakes, was not detected, the identified species may pose a health risk to humans, especially workers who come into direct contact with animals.