The Role of Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) in Biomarkers of Diabetic Nephropathy

Objective: This study investigates the potential role of Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) as a novel biomarker, evaluating its associations with established urinary biomarkers and glycemic control indicators in diabetes mellitus and diabetic nephropathy (DN). Methods: A cross-sectional study was conducted involving 84 participants stratified by albuminuria levels into normoalbuminuria, microalbuminuria, and macroalbuminuria groups. MANF levels were quantified using enzyme-linked immunosorbent assays (ELISA), while urinary biomarkers and HbA1C were measured using standard clinical methods. Correlation analyses and multivariate regression models were employed to assess relationships between MANF and biomarkers. Results: MANF levels demonstrated a significant upward trend across albuminuria categories, with the highest levels observed in the macroalbuminuria group (514.5±112.3 pg/mL, p