Assessment of the toxic effect and antiviral activity of a biocidal product using a parvovirus strain and cell cultures

The problem of combating infectious diseases using disinfectants has not been studied sufficiently. A significant number of the proposed drugs are toxic, immunosuppressive to the animal body, and cause long-term effects on the body. An urgent issue for solving the problem is the study and experimental substantiation of the effect on microorganisms of disinfectants, which include surface-active substances, detergents, quaternary ammonium compounds with their inherent prolonged action. The article presents the results of studies on determining the assessment of the toxic effect of the biocidal drug “Geocide” with antibacterial, disinfestation properties through the synergistic action of a quaternary ammonium compound, polymethyleneguanidine hydrochloride and synthetic pyrethroid on a model of rabbit kidney cell culture (RK-13), as well as the effect of the biocide on the titer of infectious activity under conditions of protein loading on a model of the field strain of parvovirus “Antey”. The work on determining the antiviral activity of different concentrations of “Geocid” was planned and carried out, guided by generally accepted recommendations for the use of the drug (Registration Certificate No. AB-05666-03-14: drug “Disinfectant “Geocid”). A sensitive in vitro assay capable of measuring the infectivity of a parvovirus strain was used to determine the effectiveness of different concentrations of the biocidal drug “Geocid”. The data show that the use of 0.5% solution of “Geocid” during the contact time (10 min) can significantly reduce parvovirus titers and that even lower concentrations, i.e. 0.25%, can effectively inactivate viruses provided that the contact time is increased to 30 min. The results also confirm the importance of cleaning before disinfection, since organic matter completely nullified the virucidal activity of biocidal drug “Geocid” solutions against parvovirus strains.