Sequencing Report of a Monoclonal Vasoinhibin Antibody

Background: vasoinhibin is an antiangiogenic fragment of the pituitary hormone prolactin which is of interest in diseases of the eye, the heart, and pregnancy complications. This is the first report of the sequence of a monoclonal antibody against vasoinhibin. Methods: whole transcriptome shotgun sequencing of a hybridoma cell culture producing a monoclonal anti-vasoinhibin antibody was performed and the protein sequences of the complementarity determining regions were determined by Kabat, IMGT and Chotia definitions. Results: a mouse IgM-kappa antibody was identified, and the complete protein sequences were determined. The complementarity determining regions were identified according to Kabat, IMGT, and Chotia definitions. Conclusions: the sequences of the antibody can be used for its recombinant production and antibody engineering.