Protease Production By Bacillus Subtilis Using Fruit Peel Waste

The production of protease enzymes using microorganisms has garnered significant attention in recent years due to their wide-ranging applications in industries such as food, pharmaceutical, and biotechnology.This study investigates the potential of Bacillus subtilis to produce protease enzymes using fruit peel extract as a substrate.Fruit peels, typically discarded as waste, offer a rich source of nutrients that can be harnessed to support microbial growth and enzyme production.In this project, we optimized the fermentation conditions for protease production by Bacillus subtilis using fruit peel extract.The effects of pH, temperature, and substrate concentration on enzyme production were evaluated.Our results showed that Bacillus subtilis produced significant amounts of protease enzymes when grown on fruit peel extract, with optimal production observed at pH 7.0, 37C, and 2% substrate concentration.The crude enzyme extract exhibited promising proteolytic activity, with potential applications in various industrial processes.This study demonstrates the feasibility of utilizing fruit peel extract as a renewable substrate for protease production, contributing to a more sustainable and environmentally friendly approach to enzyme production.