Antioxidant efficacy of synthesized composite copper lutein nano nutraceuticals from brassica oleracea var italica

Aim: Due to its antioxidant properties, lutein scavenges reactive oxygen species, including lipid ‎peroxy radicals and singlet oxygen. Despite the therapeutic promise derived from its various ‎physiological functions, lutein's absorption and stability issues make efficient distribution ‎challenging. Nanotechnology applications in pharmaceuticals have been the subject in recent decades. Similar to nanopharmaceuticals, a new class of nanomaterials known ‎as nanonutraceuticals has evolved due to the application of nanotechnology for improved ‎nutraceutical delivery.‎ Methods: For green synthesis, B. oleracea stalks and florets were utilized individually in a ‎copper sulphate solution. The synthesized Copper nanoparticles were characterized and screened ‎for their antibacterial and anti-biofilm activities. ‎ Results: At 400μg/ml concentrations, FLNP and FNP demonstrated 92 and 88% DPPH free ‎radical scavenging, respectively. Comparing the different NPs to the positive control, FLNP ‎demonstrated the highest H2O2 scavenging activity (IC50 72.55μg/ml), followed by FNP. ‎When compared to the positive control, FLNP, FNP, and Lutein demonstrated antioxidant ‎capacities of 94, 91, and 87% at 400µg/ml using the reducing power method. According to our ‎ferrozine assay results, FLNP demonstrated an efficient ability to bind iron, suggesting that this ‎property may be linked to its antioxidant properties. According to their different antioxidant ‎capacities, FLNP and FNP demonstrated 92 and 88% FZ reduction ability. ‎ Conclusion: The present study was successful in synthesizing composite lutein-based copper ‎nanoparticles and also confirmed of the potential antioxidant activity exhibited by the ‎composite particles. The composite particles did exhibit enhanced antioxidant activity when ‎compared to other controls‎.