Mycobacterium avium DNA extraction: Implications for NTM identification and amplicon sequencing
The findings demonstrate that the currently used DNA extraction method, GenoLyse<sup>®</sup> Version 1.0, remains the fastest, simplest, and most cost-effective approach for routine NTM identification, despite lower DNA yields. GenoLyse also shows potential for implementation in amplicon-based sequencing of NTM, specifically when amplifying the 16S rRNA gene.
Authors
- Janré Steyn (ORCID: https://orcid.org/0000-0003-4475-1490)
- Brendon C. Mann (ORCID: https://orcid.org/0000-0002-9658-7867)
- Robin M. Warren (ORCID: https://orcid.org/0000-0001-5741-7358)
- Christoffel J. Opperman (ORCID: https://orcid.org/0000-0002-6676-5431)
- Wynand J. Goosen (ORCID: https://orcid.org/0000-0001-6614-9084)
- Salim Ben Amor (ORCID: https://orcid.org/0009-0007-8385-4907)
- Yonas Ghebrekristos (ORCID: https://orcid.org/0000-0003-2002-5240)
- Sarishna Singh (ORCID: https://orcid.org/0000-0002-1794-4126)
- Greshan Kisten (ORCID: https://orcid.org/0009-0003-8287-902X)
Institutions
- National Health Laboratory Service (ZA)
- University of Cape Town (ZA)
- Stellenbosch University (ZA)
Publication Details
- Journal
- Southern African Journal of Infectious Diseases
- Published
- 2025-05-27
- DOI
- https://doi.org/10.4102/sajid.v40i1.717
- Primary Topic
- Mycobacterium research and diagnosis
- Type
- article
- Field-Weighted Citation Impact
- 0.00