Effects of Uridine and Uridine Nucleotides on Proliferation and Migration of L929 Murine Fibroblast Cell Line

The present study aimed to investigate the effects of uridine and the uridine nucleotides uridine-5’-monophosphate (UMP), uridine-5’-diphosphate (UDP) and uridine-5’-triphosphate (UTP) at different concentrations (1, 10 and 100 μM) on cell viability and migration capacity using the L929 murine fibroblast cell line. To assess cytotoxicity and cellular proliferation, the MTT [(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)] assay was performed at 24, 48 and 72 hours of incubation, providing a quantitative evaluation of metabolic activity and cell survival. Additionally, the scratch assay was employed and followed up to 96 hours to analyze fibroblast migration, offering insights into the role of these pyrimidines in wound healing and tissue repair. The MTT assay revealed that the highest concentration (100 μM) of UMP or UDP significantly enhanced proliferation of cells at 72 hours while uridine at 10 and 100 μM and UTP at all concentrations tested provided the same effect. In scratch assay UMP or UDP did not cause any significant cell migration while uridine and UTP, at all concentrations, significantly enhanced migration of fibroblast cells at 96 hours. The results demonstrated distinct effects of uridine and its nucleotides on cell viability and migration, with significant benefit in terms of wound healing provided by uridine and UTP, highlighting their potential biological significance and therapeutic implications in regenerative medicine and tissue engineering.