Biologically‐Inspired Melt Electrowriting for the Generation of Highly Biomimetic Functional Myocardium
Abstract In the heart, the specific 3D structure of myocardial layers produces an efficient ejection of blood. When myocardial infarction strikes, this architecture is disrupted, adding a disarranged contraction to the decreased availability of pumping units (cardiomyocytes, CMs). In this work, the alignment of cardiac fibers in a large animal model (pig) is characterized and employ melt electrowriting (MEW) to fabricate a bio‐inspired scaffold with diamond‐shaped pores. Using human‐induced pluripotent stem cell‐derived CMs and cardiac fibroblasts, human cardiac tissues with a biomimetic in‐plane contraction are generated. MEW‐diamond tissues beat macroscopically for over 1 month, with significantly faster kinetics, increased force, and higher conduction velocity than those based on square or rectangular pores. The diamond design induces a specific hiPSC‐CM alignment resulting in the observed in‐plane contraction. Transcriptomic analysis using bulk RNA‐seq reveals diamond‐MEW tissues present features of maturation as compared to traditional 2D cultures. Finally, the bio‐inspired cardiac tissues are employed to treat an infarction model in athymic rats, showing a significant benefit on systolic function and remodeling, tied to the presence of large grafts of human cells remuscularizing the ventricular wall. All in all, it is demonstrated that the new design generates superior human cardiac tissues with therapeutic capacity.
Authors
- Asier Ullate‐Agote (ORCID: https://orcid.org/0000-0002-8595-7703)
- Eduardo Larequi (ORCID: https://orcid.org/0000-0003-3665-3073)
- Ricardo M Rosales (ORCID: https://orcid.org/0000-0002-8867-3802)
- Felipe Prósper (ORCID: https://orcid.org/0000-0001-6115-8790)
- Gloria Abizanda (ORCID: https://orcid.org/0000-0002-2504-5163)
- Elena Iglesias (ORCID: https://orcid.org/0000-0002-8956-4843)
- María Flandes‐Iparraguirre
- Nicolás Laita
- Pilar Montero‐Calle
- Xabier L. Aranguren (ORCID: https://orcid.org/0000-0002-1834-603X)
- Olalla Iglesias‐García (ORCID: https://orcid.org/0000-0002-7728-9207)
- Paula Aguirre‐Ruiz (ORCID: https://orcid.org/0000-0002-5871-372X)
- Aida Oliván‐Viguera (ORCID: https://orcid.org/0000-0001-5348-924X)
- Patxi San Martín‐Úriz (ORCID: https://orcid.org/0000-0003-1483-4279)
- Esther Pueyo (ORCID: https://orcid.org/0000-0002-1960-407X)
- Elena M. De‐Juan‐Pardo (ORCID: https://orcid.org/0000-0001-9005-7497)
- Estefanía Peña
- Manuel M. Mazo (ORCID: https://orcid.org/0000-0002-4579-7741)
- M. Doblaré (ORCID: https://orcid.org/0000-0001-8741-6452)
- Miguel Ángel Martínez
- Juán José Gavira
- Andrea Sánchez‐Bueno
- Ilazki Anaut‐Lusar
Institutions
- Nanjing Tech University (CN)
- The University of Western Australia (AU)
- Universidad de Zaragoza (ES)
- Harry Perkins Institute of Medical Research (AU)
- Queen Elizabeth II Medical Centre (AU)
- Navarre Institute of Health Research (ES)
- Clinica Universidad de Navarra (ES)
- Instituto de Investigación Sanitaria Aragón (ES)
- Centro de Investigación Biomédica en Red de Cáncer (ES)
- Universidad de Navarra (ES)
Publication Details
- Journal
- Advanced Functional Materials
- Published
- 2025-04-18
- DOI
- https://doi.org/10.1002/adfm.202420106
- Citations
- 7
- Primary Topic
- Neuroscience and Neural Engineering
- Type
- article
- Field-Weighted Citation Impact
- 6.36
Funders
- Biogen
- Ministerio de Ciencia, Innovación y Universidades
- European Commission
- Gobierno de Aragón
- Gobierno de Navarra
- Horizon 2020 Framework Programme
- Agencia Estatal de Investigación