Advances and Challenges in Fluorescence‐Based Biosensors for Biological and Chemical Detection

Abstract Fluorescence‐based biosensors have become highly effective analytical instruments, revolutionizing the landscape of biological and chemical detection across diverse fields. These devices harness the unique properties of fluorescence to detect and quantify a wide array of analytes with remarkable sensitivity and specificity. By integrating biological recognition elements with advanced optical technologies, fluorescence‐based biosensors provide high sensitivity, rapid detection, and real‐time monitoring capabilities for diagnostic applications. This review discusses the fundamental principles of fluorescence biosensing, its key advantages, and the various types. The applications of these sensors span fields such as medicine, monitoring of the environment, food safety, biosecurity, and agriculture. Although fluorescence biosensors offer numerous benefits, the review also addresses the ongoing challenges and limitations researchers are working to overcome, such as photobleaching, background autofluorescence, and limited tissue penetration depth. The review emphasizes the critical design considerations and the continuous advancements in developing these innovative analytical tools, which are poised to transform the future of biological and chemical detection.