Pseudouridine synthases are proviral factors for Sindbis virus in insect and mammalian cells
Alphaviruses pose a threat to over half of the global population, and currently, there are no approved antivirals targeting alphaviruses. We identified a conserved pseudouridine synthase that is proviral for Sindbis virus (SINV) infection in insects and humans. Using Psi-seq, we identified putative pseudouridine residues in SINV RNA. Mutagenesis of putative psi sites led to a slight reduction in replication and suggests that pseudouridine residues in SINV RNA are functionally important in replication.
Authors
- Ram Podicheti (ORCID: https://orcid.org/0000-0003-3449-5155)
- Douglas B. Rusch (ORCID: https://orcid.org/0000-0002-1066-2687)
- Irene L. G. Newton (ORCID: https://orcid.org/0000-0002-7118-0374)
- N. Stark
- Richard W. Hardy (ORCID: https://orcid.org/0000-0001-6912-6291)
- Laura García (ORCID: https://orcid.org/0000-0001-9424-9031)
- Adela Krenz
Institutions
- Indiana University Bloomington (US)
- Indiana University (US)
Publication Details
- Journal
- mBio
- Published
- 2025-05-27
- DOI
- https://doi.org/10.1128/mbio.01329-25
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health
- National Science Foundation of Sri Lanka