YIPF5 is an essential host factor for porcine epidemic diarrhea virus double-membrane vesicle formation
Coronaviruses pose serious health threats to both humans and animals. Identifying host genes critical for porcine epidemic diarrhea virus (PEDV) infection can uncover new therapeutic targets and enhance our understanding of coronavirus pathogenesis. In this study, we conducted genome-scale CRISPR/Cas9 screens in two porcine cell lines (IPEC-J2 and IPI-2I) and identified YIPF5 as an essential host factor for PEDV replication. Our results demonstrate that YIPF5 plays a pivotal role in the formation of PEDV-induced double-membrane vesicles (DMVs), which are crucial for viral replication. These findings shed new light on the molecular mechanisms of PEDV and suggest YIPF5 as a therapeutic target.
Authors
- Zhuolin Hao
- Yuhai Bi (ORCID: https://orcid.org/0000-0002-5595-363X)
- Xiaoyue Duan (ORCID: https://orcid.org/0000-0002-5792-2335)
- Sen Wu (ORCID: https://orcid.org/0000-0003-0764-6122)
- Xuguang Du (ORCID: https://orcid.org/0000-0001-9032-8330)
- Jin Li (ORCID: https://orcid.org/0000-0001-9201-2321)
- Liang Guo (ORCID: https://orcid.org/0000-0002-4988-9993)
- Yun Chen (ORCID: https://orcid.org/0000-0001-6819-6562)
Institutions
- Czech Academy of Sciences, Institute of Microbiology (CZ)
- Sanya University (CN)
- Early Warning (United States) (US)
- University of Chinese Academy of Sciences (CN)
- China Agricultural University (CN)
Publication Details
- Journal
- Journal of Virology
- Published
- 2025-05-27
- DOI
- https://doi.org/10.1128/jvi.00320-25
- Citations
- 2
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 2.39
Funders
- National Key Research and Development Program of China