Comparative Analytical Methods for the Simultaneous Estimation of Olmesartan and Chlorthalidone in Formulation Dosage Form Using UV Spectroscopy and RP-HPLC

Hypertension (HTN) affects millions globally, necessitating effective treatment strategies often involving combination drug therapy.This study focuses on developing and validating analytical methods for the simultaneous estimation of Olmesartan (OLM) and Chlorthalidone (CHL), two drugs frequently combined for hypertension management.Two methods were explored: UV spectrophotometry and High-Performance Liquid Chromatography (HPLC).The main aim is to compare the assay results for the above said combination using two a For the UV method, methanol and distilled water served as solvents, with OLM and CHL showing absorbance maxima at 258 nm and 214 nm, respectively.The method demonstrated linearity within 20-60 g/ml for OLM and 2-10 g/ml for CHL, with excellent precision, accuracy, and sensitivity.The HPLC method utilized a mobile phase of methanol, acetonitrile, and orthophosphoric acid, achieving separation on a C18 column with UV detection.Calibration curves for OLM and CHL displayed linearity over the same concentration ranges.Both methods were rigorously validated, showing robustness across varied parameters, including flow rates and mobile phase composition.This study provides reliable, efficient methodologies for the simultaneous analysis of OLM and CHL in pharmaceutical formulations, supporting enhanced quality control and patient adherence in antihypertensive therapy.