SesZen-Bio: A Novel Modulator of Wnt/β-Catenin Signalling for Promoting Hair Growth and Follicular Regeneration

SesZen-Bio TM , a proprietary extract, is obtained from Sesbania grandiflora leaves and is implicated in having a beneficial effect on hair growth but its mechanism of action is unknown.The present study aims to evaluate the SesZen-Bio TM effects on the growth and proliferation of Outer Root Sheath Cells and Dermal Papilla cells.SesZen-Bio TM effect on in vitro hair cell proliferation was evaluated by MTT assay and metabolic activity of DPCs by cell viability ATP assay.To characterize and further understand the mechanism of action of ORSCs and DPCs, gene expression analysis was performed by Real-time polymerase chain reaction .Hair growth analysis was performed using ex-vivo hair culture (Philpott test).The isolated ORSCs were shown to express CK5, CK10, and CK15 and DPCs expressed BMP4, Vcan, and Lef1.The treatment with 10 g/ml SesZen-Bio TM resulted in an increase in ORSCs proliferation, an increase in Wnt, -catenin, Sox9, and CK15 expression respectively, and ATP production in DPCs, compared to the respective control group.The ex vivo organ culture of a complete hair strand showed that ten days of 10 g/ml SesZen-Bio TM treatment significantly increased hair shaft length when compared to the control group.SesZen-Bio TM increases hair cell growth in vitro and ex vivo possibly by activation of the Wnt/-catenin signalling and stem cell differentiation pathways.These favorable results indicate that the formulation can be used as a potential candidate for hair growth formulation.