UV-Spectrometric Method Development and Validation of Hydrochlorothiazide in the Bulk and Pharmaceutical Dosage Form

Validation is a documented program that provides a high degree of assurance that a facility or operation will consistently produce product meeting a predetermined specification.The present research work is mainly emphasized to establish a novel, simple, economic, rapid and accurate method for the estimation of hydrochlorothiazide drug by UV-spectrophotometric method.The method was successfully developed by using distilled water as a mobile phase and the maximum absorbance was found to be at 272 nm.To confirm the developed method's appropriateness, it was validated as per ICH guidelines.This method obeyed Beers-Lambert's law over a concentration range of 5 to 17.5 g/ml.The method was confirmed to be accurate with good recovery value and precise showing % RSD value within acceptance criteria.Due to its rapidness, accuracy, precision, sensitivity, ruggedness and more affordable mobile phase confirmed that this method can be undoubtedly applicable for routine quality control analysis of hydrochlorothiazide in bulk and pharmaceutical dosage form.