Optimized production of bacterial nanocellulose by <i>Komagataeibacter xylinus</i> HBCM‐B0092 and preliminary application with sugarcane bagasse

Abstract Production of bacterial nanocellulose (BNC) from lignocellulose biomass has attracted attention as a potential and sustainable approach in supplying nanocellulose for various applications. This study of BNC production was involved optimizing the culture conditions for Komagataeibacter xylinus HBCM‐B0092 with glucose as a carbon source and investigating its BNC synthesis using sugarcane bagasse hydrolysate as a substitute for glucose. To optimize BNC production from Komagataeibacter xylinus HBCM‐B0092, a Box–Behnken design was employed to investigate the effects of glucose concentration, pH, and culture surface area on BNC yield. Optimal conditions were determined to be 20.5 g/L glucose, pH 5.0, and a surface area of 58 cm 2 , resulting in a BNC production yield of 9.43%. Sugarcane bagasse hydrolysate was utilized as a carbon source for BNC production to explore the potential of sustainable feedstock. Hydrothermal hydrolysis of sugarcane bagasse yielded a hydrolysate containing approximately 17.0 g/L of reducing sugars. Fermentation of this hydrolysate with different initial sugar concentrations (15, 20, and 25 g/L) resulted in BNC yields ranging from 1.92 to 3.62% at pH 5.0. SEM analysis revealed no significant differences in the fiber structure and diameter of the BNC produced from glucose and sugarcane bagasse hydrolysate. XRD analysis indicated a high crystallinity index of up to 52.9% for bagasse‐derived BNC. FTIR analysis showed similar spectral profiles for both types of BNC. This study successfully optimized BNC production using K. xylinus HBCM‐B0092 and demonstrated the feasibility of utilizing sugarcane bagasse as a sustainable alternative to traditional carbon sources. This approach offers a promising avenue for the production of environmentally friendly and high‐value BNC materials.