Pertumbuhan Plantlet Anggrek Dendrobium Discolor ‘Merauke’ pada Media Vacint and Went dengan Pengaturan Dosis Salicylic Acid dan Activated Charcoal

Indonesia has high biological wealth, one of which is the Dendrobium discolor 'Merauke' orchid endemic to the Papuan forest. The use of in vitro culture techniques with Vacin and Went media, supplemented with salicylic acid and activated charcoal, is believed to enhance growth and reduce contamination in in vitro culture media. This study aims to determine the interaction and dosage between salicylic acid and activated charcoal on the growth of Dendrobium discolor 'Merauke' orchid plantlets. 2-factorial Completely Randomized Design (CRD) with 4 replications was used, followed by analysis using 5% DMRT. The parameters of this study included leaf length, leaf width, number of leaves, root length, and number of roots. A dose of 50 ppm salicylic acid combined with various doses of activated charcoal resulted in the longest leaves, while a dose of 75 ppm salicylic acid without activated charcoal produced the widest leaves. The treatment with 1 g of activated charcoal without salicylic acid led to the longest roots, whereas the treatment with 3 g/l of activated charcoal without salicylic acid resulted in the highest number of roots.