A CRISPR-Cas9 system for knock-out and knock-in of high molecular weight DNA enables module-swapping of the pikromycin synthase in its native host
Our gene-editing tool has enabled the efficient replacement of extensive and repetitive DNA regions within streptomycetes.
Authors
- Zhe‐Chong Wang
- Adrian T. Keatinge‐Clay (ORCID: https://orcid.org/0000-0002-4358-7628)
- Takeshi Miyazawa
- Hayden Stegall
Institutions
- The University of Texas at Austin (US)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-05-27
- DOI
- https://doi.org/10.1186/s12934-025-02741-w
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- University of Texas at Austin
- National Institutes of Health
- National Institute of General Medical Sciences