EBiS spectra in total DNA samples carrier different single nucleotide polymorphisms of the enzyme 8-Oxoguanine DNA Glycosylase 1 (OGG1)

Abstract Techniques for quantification of Deoxyribonucleic Acids (DNA) and detection of genes are of vital importance in the evaluation of certain pathologies for early diagnosis and treatment. Current molecular biology techniques require specialized and high-cost equipment. Electrical Bioimpedance Spectroscopy (EBiS) is an emerging technique that has demonstrated sensitivity for the detection of different concentrations of DNA. Single nucleotide polymorphisms (SNP) of the enzyme 8-Oxoguanine DNA Glycosylase 1 (OGG1) (Ser326Ser, Ser326Cys, Cys326Cys) have been associated to oxidative DNA damage considered an important contributor in male infertility. SNPs identification and detection required sophisticated techniques such as DNA Sequencing and Polymerase Chain Reaction (PCR) respectively. In this work EBiS measurements were used to explore its potential value to detect SNP of the OGG1 in total DNA. Total DNA was extracted in 29 semen samples of infertile males, and EBiS measurements were developed in the frequency range of 0.01 to 1 MHz. The polymorphism presence was confirmed through nucleotide sequencing and PCR final point evidenced in agar gel. Bioimpedance spectra magnitude at low frequency seems to have enough sensitivity to distinguish polymorphism CC with respect to CG and GG. EBiS exhibited potential for detection of different oxidative damage of total DNA samples between genotypes.