Detection of Dengue and Chikungunya Viruses in Mosquitoes from Nepal

The dengue virus (DENV) and chikungunya virus (CHIKV) infection present with similar symptoms and are transmitted by Aedes mosquitoes. Until now, there have been no reported cases of DENV and CHIKV being detected in mosquitoes in Nepal. This study identified circulating arboviruses from mosquitoes collected in the capital city Kathmandu, and Dhading, Bhaktapur, and Lalitpur districts of Central Nepal in 2023. Of 105 Aedes pooled samples, 16 (15.2%) were positive for DENV, and six (5.7%) were positive for CHIKV by quantitative real-time polymerase chain reaction. DENV-2 was the most common serotype (11.4%), aligning with recent outbreaks in Nepal in 2023, followed by DENV-3 and DENV-4 (1.9% each). To the best of our knowledge, our study is the first report to detect DENV and CHIKV in Aedes mosquitoes circulating in Nepal. This study highlights the need for mosquito surveillance to prevent future outbreaks of mosquito-borne diseases in Nepal.

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Publication Details

Journal
American Journal of Tropical Medicine and Hygiene
Published
2025-05-27
DOI
https://doi.org/10.4269/ajtmh.24-0685
Citations
1
Primary Topic
Mosquito-borne diseases and control
Type
article
Field-Weighted Citation Impact
1.88

Funders

Controls
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article

Detection of Dengue and Chikungunya Viruses in Mosquitoes from Nepal

Takeshi Urano, Mya Myat Ngwe Tun, Sandesh Rimal, Satoshi Kaneko et al.
1 citations
American Journal of Tropical Medicine and Hygiene
Mosquito-borne diseases and control
1.88
article

Detection of Dengue and Chikungunya Viruses in Mosquitoes from Nepal

Takeshi Urano, Mya Myat Ngwe Tun, Sandesh Rimal, Satoshi Kaneko, Sabin Shrestha, Merveille Kapandji, Shyam Prakash Dumre, Yuki Takamatsu, Himal Luitel, Kouichi Morita, Suprabha Subedi, Basu Dev Pandey, Anjana Kharbuja, Renu Bhandari Dumre, Ishan Gautam, Anjana Khadka, Shingo Inoue, Maurine Mumo Mutua, Anjali Dulal
article en
1 citations

Abstract

The dengue virus (DENV) and chikungunya virus (CHIKV) infection present with similar symptoms and are transmitted by Aedes mosquitoes. Until now, there have been no reported cases of DENV and CHIKV being detected in mosquitoes in Nepal. This study identified circulating arboviruses from mosquitoes collected in the capital city Kathmandu, and Dhading, Bhaktapur, and Lalitpur districts of Central Nepal in 2023. Of 105 Aedes pooled samples, 16 (15.2%) were positive for DENV, and six (5.7%) were positive for CHIKV by quantitative real-time polymerase chain reaction. DENV-2 was the most common serotype (11.4%), aligning with recent outbreaks in Nepal in 2023, followed by DENV-3 and DENV-4 (1.9% each). To the best of our knowledge, our study is the first report to detect DENV and CHIKV in Aedes mosquitoes circulating in Nepal. This study highlights the need for mosquito surveillance to prevent future outbreaks of mosquito-borne diseases in Nepal.

American Journal of Tropical Medicine and HygieneVol. 113(2)
Tribhuvan University (NP), Shimane University (JP), Agriculture and Forestry University (NP), Kenya Medical Research Institute (KE), Nagasaki University (JP), Kathmandu University (NP)
Japan Agency for Medical Research and Development, Tribhuvan University, Shimane University, Japan Society for the Promotion of Science
Good health and well-being
Openalex Percentile: Top 15%
Mosquito-borne diseases and control
1.88
DOIOpen Access PDFOpenAlex
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