The study of anti-inflammatory action of various remedies in the treatment of chronic periodontitis

Objective. To study the anti-inflammatory effects of the “Alvostaz-sponge” medical product for the treatment of chronic periodontitis. Materials and methods. The culture of juvenile human dermal fibroblasts was treated with TNF-α (1st series of experiments) and a mixture of TNF-α with “Alvostaz-sponge” extract (2nd series of experiments). The concentration of cytokines IL-6, MCP-1 in the culture medium of fibroblasts was assessed using enzyme immunoassay (ELISA). The examination and treatment of 74 patients (49 women and 25 men), with a diagnosis of chronic generalized periodontitis of moderate severity were carried out. Results. In response to TNF-α stimulated inflammation, fibroblasts sharply increased the production of both chemokine MCP-1 and the inflammatory mediator IL-6 into the culture medium. A dose-dependent rise of “Alvostaz-sponge” extract content in the culture medium correlated with a decrease in the concentration of MCP-1, which indicates a pronounced inhibitory effect of “Alvostaz-sponge” extract on the production of MCP-1 cytokine. An increase in the concentration of IL-6 correlated with a dose-dependent increase in the extract of “Alvostaz sponge” in the culture medium of TNF-α-stimulated fibroblasts. Conclusions. The results of the study demonstrated “Alvostaz-sponge” medication to be a powerful inhibitor of chemokine MCP-1 production and, it is due to this effect that it restricts the monocyte cells migration into the periodontal pocket zone. The obtained results of the treatment show accelerated healing of the periodontal complex when using the “Alvostaz-sponge” remedy.