Study of the influence of "Klyuchi" mineral water on the representatives of the indigenous intestinal microbiota

Objective. To study the bacteriotropic properties of "KLYUCHI" mineral water in relation to bacteria of the genera Bifidobacterium, Lactobacillus and Escherichia. Materials and methods. The impact of "KLYUCHI" mineral water on the representatives of the indigenous microbiota of the gastrointestinal tract was determined. Probiotic strains L. plantarum 8P-A3 and B. bifidum 1 were cultivated on a "starvation" nutrient medium. The level of accumulation of cellular biomass, pH, acid-forming activity and concentration of the carbohydrate component were assessed in the culture fluid, The influence of mineral water on the physiological state of bacterial cells was studied by determining the intensity of bioluminescence of the genetically engineered strain E. coli lum+ using various solvents for rehydration of the lyophilized indicator culture. Results. "KLYUCHI" mineral water had a significant stimulating effect on the accumulation of biomass and acid formation of the tested strains of lactobacilli and bifidobacteria. The stimulating effect was more pronounced in the bifidobacteria culture. Contact of bacterial cells with mineral water both in the form of a solvent for the lyophilized culture and as an additional component of the nutrient medium provided almost equivalent results. Mineral water had a pronounced stimulating effect on the bioluminescence of the indicator strain, increasing the intensity of its luminescence twice compared to the control. Conclusions. The stimulating effect of “KLYUCHI” mineral water on the representatives of the indigenous intestinal microbiota confirms the validity of its use in the treatment of gastrointestinal diseases, eating and metabolic disorders, as well as in probiotic therapy.