Chemical composition of the endogenous fungus <i>Fusarium</i> sp. L-3 derived from Chinese Qinan agarwood

One undescribed compound named fusagarone A (<b>1</b>) and ten secondary metabolites (<b>2</b>-<b>11</b>) were isolated from the endophytic fungus <i>Fusarium</i> sp. L-3 derived from Chinese agarwood 'Qi-Nan'. The structure of <b>1</b> was elucidated by NMR spectroscopy as well as HRESIMS data. All compounds were measured for anti-inflammatory, cytotoxicity activity and <i>α</i>-glucosidase inhibitory activities. Among them, compound <b>11</b> potently suppressed nitric oxide (NO) production on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages with IC₅₀ value of 4.78 <i>μ</i>M. Moreover, compound <b>11</b> exhibited potent inhibitory effect on <i>α</i>-glucosidase with IC₅₀ value of 42.88 <i>μ</i>M, which was further clarified by means of the enzyme kinetic analysis and molecular docking simulations.