Decalin derivatives from an endophytic fungus <i>Trichoderma harzianum</i> GLQ03 and their anti-inflammatory activities

Eight metabolites (<b>1</b>-<b>8</b>) including a new compound tandyukisin J (<b>1</b>) were isolated from <i>Trichoderma harzianum</i> GLQ03 isolated from <i>Ganoderma lucidum</i>. Their absolute configurations were determined based on spectroscopic analysis and CD comparison. In this study, specific decalin derivatives (compounds <b>2</b>, <b>3</b>, <b>4</b>, <b>6</b>, <b>7</b>, and <b>8</b>) were found to significantly inhibit IL-6/IL-1β expression using RT-qPCR in an LPS-induced RAW264.7 macrophage inflammatory model. These results provide the molecular basis of the anti-inflammatory activity of decalin compounds.