Microparticle Bombardment as a Method for Transgenesis in Auanema and Tokorhabditis
Functional gene analysis tools in <i>Caenorhabditis elegans</i> are often ineffective in other nematodes due to differences in gonadal morphology and transgene silencing. Here, we established a method to generate stable transgenic lines in the nematodes <i>Auanema freiburgense</i> and <i>Tokorhabditis tufae</i> using microparticle bombardment coupled with hygromycin B selection. Despite using non-codon-optimized GFP, transgenic strains expressing fluorescent markers were obtained in both species. Additionally, an <i>Auanema</i> codon-optimized RFP construct showed robust expression in all tissues. This method will be valuable for future studies into the unusual sex determination, viviparity, and stress resistance in <i>Auanema</i> and <i>Tokorhabditis</i> .
Authors
- Tatsuya Yamashita
- Asako Sugimoto (ORCID: https://orcid.org/0000-0001-6001-4293)
- Ryoji Shinya (ORCID: https://orcid.org/0000-0002-2450-3054)
- Taisei Kikuchi (ORCID: https://orcid.org/0000-0003-2759-9167)
- Sally Adams (ORCID: https://orcid.org/0000-0002-3353-7459)
- Masahiko Hasumi (ORCID: https://orcid.org/0000-0002-2273-6292)
- Nami Haruta (ORCID: https://orcid.org/0000-0002-4232-9635)
- Takae KUSANO
- Shun Oomura (ORCID: https://orcid.org/0000-0001-9270-2541)
- André Pires‐daSilva (ORCID: https://orcid.org/0000-0002-0741-7197)
Institutions
- Meiji University (JP)
- Tohoku University (JP)
- The University of Tokyo (JP)
Publication Details
- Journal
- PubMed
- Published
- 2025-03-23
- DOI
- https://doi.org/10.17912/micropub.biology.001585
- Citations
- 1
- Primary Topic
- RNA Interference and Gene Delivery
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Leverhulme Trust
- Biotechnology and Biological Sciences Research Council
- Japan Society for the Promotion of Science
- Core Research for Evolutional Science and Technology