Design, Expression, and Purification of a Fusion Enzyme Containing Terminal Deoxynucleotidyl Transferase from B. bovis and DNA-Binding Proteins from E. coli
The fusion of TdT with EcSSB resulted in increased thermal stability and a reduced ability to add nucleotides to the substrate.
Authors
- Yaraslau Dzichenka (ORCID: https://orcid.org/0000-0002-8655-8704)
- Veronika V. Shchur (ORCID: https://orcid.org/0000-0002-2191-5707)
- А. V. Yantsevich (ORCID: https://orcid.org/0000-0001-6786-3589)
- Antos B. Sachanka (ORCID: https://orcid.org/0000-0001-8198-0909)
Institutions
- National Academy of Sciences of Belarus (BY)
- Institute of Bioorganic Chemistry (BY)
Publication Details
- Journal
- Protein and Peptide Letters
- Published
- 2025-05-26
- DOI
- https://doi.org/10.2174/0109298665372636250504084653
- Citations
- 1
- Primary Topic
- Bacteriophages and microbial interactions
- Type
- article
- Field-Weighted Citation Impact
- 1.72