Cell-Free Expression of HPV16 Minor Capsid Protein L2 and Its Interaction with S100A10
In this study, we successfully expressed the HPV16 L2 protein using a cell-free protein expression system. Preliminary structural analysis using TEM and CD revealed key structural features of L2. Furthermore, SPR analysis provided detailed kinetic parameters for its interaction with S100A10. These findings provide more details on understanding L2's structural features, with broader implications for antipathogen studies.
Authors
- Lian Wu (ORCID: https://orcid.org/0000-0003-3110-7380)
- Qingshan Fu (ORCID: https://orcid.org/0000-0002-7445-2355)
- Wenqi Jiang
- Xiangchun Shen
Institutions
- Guiyang Medical University (CN)
- Chinese Academy of Sciences (CN)
- Shanghai Institute of Materia Medica (CN)
Publication Details
- Journal
- Protein and Peptide Letters
- Published
- 2025-05-26
- DOI
- https://doi.org/10.2174/0109298665390494250513110604
- Citations
- 1
- Primary Topic
- S100 Proteins and Annexins
- Type
- article
- Field-Weighted Citation Impact
- 0.81