The Case for Limiting “Sequences of Concern” to Those with Demonstrated Pathogenic Function
Screening synthetic nucleic acid orders for sequences of concern is a necessary part of a healthy biosecurity regime, but it exacts costs for nucleic acid providers. Taxonomy is and will remain a critical part of the decision-making process for screening, especially for viral sequences. But, moving forward, the function of a sequence will also be determinative of its level of concern, or lack thereof. Stakeholders continue to debate which functions are "of concern." However these are ultimately adjudicated, nonviral sequences with unknown or hypothetical functions which, by definition, can bear no resemblance to sequences with concerning functions, must be considered innocent of harmful effects. To qualify as a nonviral sequence of concern, the sequence to which it is the best match must be demonstrated in the published literature to have a function of concern.
Authors
- Tyler S. Laird (ORCID: https://orcid.org/0000-0003-2317-2895)
- Peter A. Carr (ORCID: https://orcid.org/0000-0002-4078-8846)
- Samuel P. Forry (ORCID: https://orcid.org/0009-0000-8200-0327)
- M. T. Parker (ORCID: https://orcid.org/0000-0002-4359-8677)
- Jacob Beal (ORCID: https://orcid.org/0000-0002-1663-5102)
- Tessa Alexanian (ORCID: https://orcid.org/0000-0003-1108-7124)
- James Diggans (ORCID: https://orcid.org/0000-0003-3871-2380)
- Rebecca Mackelprang (ORCID: https://orcid.org/0000-0002-2851-3823)
- Bryan T. Gemler (ORCID: https://orcid.org/0000-0002-9635-4545)
- Nicole E. Wheeler (ORCID: https://orcid.org/0000-0003-4599-9164)
- Sheng Lin‐Gibson (ORCID: https://orcid.org/0000-0001-5092-1519)
- Todd J. Treangen (ORCID: https://orcid.org/0000-0002-3760-564X)
- Craig Bartling (ORCID: https://orcid.org/0000-0002-3040-7149)
- Kevin M. Esvelt (ORCID: https://orcid.org/0000-0001-8797-3945)
- Gene D. Godbold (ORCID: https://orcid.org/0000-0002-5702-4690)
- Caitlin Jagla (ORCID: https://orcid.org/0000-0002-6139-8150)
- Gregory D. Koblentz (ORCID: https://orcid.org/0000-0002-0793-1209)
- Joan E Rodríguez Gil (ORCID: https://orcid.org/0009-0001-3989-967X)
- Krista L. Ternus (ORCID: https://orcid.org/0000-0003-1138-5308)
- India Hook-Barnard (ORCID: https://orcid.org/0000-0002-7103-2152)
- Kevin Flyangolts (ORCID: https://orcid.org/0009-0000-5232-9615)
- Kemper Talley
- Jens Berlips (ORCID: https://orcid.org/0009-0007-3429-9265)
Institutions
- Association for Computing Machinery (US)
- Battelle (US)
- International Baccalaureate (CH)
- George Mason University (US)
- Georgetown University (US)
- Kennedy Center (DK)
- Twist Bioscience (United States) (US)
- Buckingham Browne & Nichols (US)
- Clinical Research Consortium (US)
- Signature Research (United States) (US)
- Massachusetts Institute of Technology (US)
- Rice University (US)
- University of Birmingham (GB)
- Swiss National Science Foundation (CH)
Publication Details
- Journal
- Applied Biosafety
- Published
- 2025-05-26
- DOI
- https://doi.org/10.1089/apb.2025.0015
- Citations
- 3
- Primary Topic
- Zoonotic diseases and public health
- Type
- article
- Field-Weighted Citation Impact
- 5.64