FEATURES OF P53 AND VEGF EXPRESSION IN TUMOR TISSUE DURING BREAST CANCER PROGRESSION

The purpose – to study the features of p53 and VEGF protein expression in tumor tissueduring breast cancer progression after complex treatment, depending on the stage of theoncological process and the molecular type of the tumor.Material and methods. 148 women with breast cancer of stages I-III C were examined, fromwhom samples of tumorous breast tissue were taken. Biopsy material was stored in paraffi nblocks. 5 years after the comprehensive treatment of these women, the biopsy material wasdivided into two groups. The main group included 69 samples of biopsy material takenfrom women with verifi ed progression of breast cancer, the comparison group included 79samples of biopsy material from women without progression of breast cancer.Both groups under study were representative in terms of age, stage of the disease andmolecular type of the tumor, performed treatment.Immunohistochemical study of p53 and angiogenesis marker VEGF was carried outusing the imaging system («DaKo Cytomation», Denmark) depending on the stage of thebreast cancer and its molecular type. The results of the immunohistochemical reactionfor the detection of VEGF and p53 were evaluated by a semi-quantitative method, bydetermining the number of positively stained cells- label index (MI, %). Results. In both groups under study, an increase in the expression of p53 and VEGF in the tumor tissue is noted with each subsequent stage of the oncological process, that indicates a clear dependence of the expression of the above- mentioned proteins and the breast cancer stage. This dependence is especially observed in the VEGE expression in the group of comparison, since the dynamics is veritable at each stage. In both study groups, p53 and VEGF expression signifi cantly predominated in triple- negative tumor types compared to Luminal- A, Luminal- B, Her/2new+ types.The above- mentioned proteins, in particular VEGF, can be used as markers for the prognosis of the course of breast cancer after complex treatment.Conclusions. 1. There is a dependence of the expression of p53 and VEGF proteins in tumor tissue on the stage of breast cancer, as well as the molecular type of the tumor. 2. Breast cancer progression after complex treatment is characterized by an increase in the expression of p53 and VEGF proteins in tumor tissue at each subsequent stage of the oncological process. 3. In both study groups, the expression of p53 and VEGF is signifi cantly higher in triple- negative tumors compared to Luminal- A, Luminal- B, Her/2new+ types.