A dual‐probe one‐pot <scp>RPA</scp>‐<scp>CRISPR</scp>/Cas12a: a highly sensitive and rapid method for detection of <scp><i>Phytophthora vignae</i></scp>
This study developed a dual-probe one-pot RPA-CRISPR/Cas12a detection platform that demonstrates rapid and highly sensitive identification of P. vignae. The proposed technology enables field-deployable detection without requiring specialized technical expertise, representing a promising approach with substantial potential for practical applications. © 2025 Society of Chemical Industry.
Authors
- Qinghe Chen (ORCID: https://orcid.org/0000-0002-1797-7614)
- Zhiming Zhou
- Zhiting Li (ORCID: https://orcid.org/0000-0002-0854-9538)
- Zaobing Zhu (ORCID: https://orcid.org/0000-0001-7290-2132)
- Wanzhen Feng
- Chengdong Yang (ORCID: https://orcid.org/0009-0007-1678-8380)
- Mingze Lin
Institutions
- Hainan University (CN)
- Jiangsu Academy of Agricultural Sciences (CN)
- Institute of Plant Protection (CN)
- Sanya University (CN)
Publication Details
- Journal
- Pest Management Science
- Published
- 2025-05-26
- DOI
- https://doi.org/10.1002/ps.8932
- Citations
- 2
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- Hainan University
- National Key Research and Development Program of China