Research Progress on Prokaryotic and Eukaryotic Expression Systems in Fish

构建功能蛋白高效表达系统，在鱼类人工养殖领域具有潜在的应用价值。重组蛋白获取是通过在宿主细胞中引入特定基因以表达目标蛋白来实现的，这一过程涉及基因克隆、载体构建、宿主细胞选择、蛋白表达和纯化等多个步骤。本文综述了常用的重组蛋白表达系统(如大肠杆菌、酵母、昆虫细胞和哺乳动物细胞)的潜力和局限性，以及不同表达系统在生产重组蛋白中的应用，旨在为鱼类重组蛋白的研究与应用提供重要参考。The construction of efficient expression systems for functional proteins has potential applications in the aquaculture field. Recombinant proteins are synthesized by introducing specific genes into host cells to achieve targeted protein expression, a process encompassing multiple steps such as gene cloning, vector construction, host cell selection, protein expression, and purification. This article reviews the strengths and limitations of commonly used recombinant protein expression systems in fish (e.g., Escherichia coli, yeast, insect cells, and mammalian cells), as well as the applications of different expression systems in producing recombinant proteins, aiming to provide critical insights for the research and application of recombinant proteins in fish.