Study on the Effect of Tea Saponin on Fungal Liquid Fermentation to Produce Cellulase and Its Enzymatic Characteristics

本实验对厚垣镰孢霉HML278采用液体发酵产纤维素酶，加入0.2%、0.4%、0.6%、0.8%、1.0%不同浓度的茶皂素，探究茶皂素对其产纤维素酶的影响。实验结果显示，加入0.6%浓度的茶皂素的纤维素酶活性最高。在酶学特性研究中，酶的最适温度为60℃，最适pH为6.4。在金属离子对酶活性的探究中，发现Li+、K+、Ca2+、Mn2+、Zn2+、Fe3+对酶促反应具有促进作用，其中Ca2+、Mn2+对酶促反应的促进作用最强；而Co2+、Al3+对酶促反应具有抑制作用。This experiment used liquid fermentation to produce cellulase from Fusarium graminearum HML278, and added tea saponin at different concentrations of 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% to investigate the effect of tea saponin on its cellulase production. The experimental results showed that the cellulase activity was highest when 0.6% concentration of tea saponin was added. In the study of enzymatic characteristics, the optimal temperature and pH for the enzyme are 60˚C and 6.4, respectively. In the exploration of metal ions on enzyme activity, it was found that Li+, K+, Ca2+, Mn2+, Zn2+, and Fe3+ have a promoting effect on enzymatic reactions, among which Ca2+ and Mn2+ have the strongest promoting effect on enzymatic reactions; Co2+ and Al3+ have inhibitory effects on enzymatic reactions.