A Mg2+-dependent high-yield method for extracting high-molecular-weight genomic DNA from a single planarian specimen
This protocol offers a simple and high-yield solution for isolating HMW gDNA from D. japonica. It also provides an alternative for organisms whose gDNA consistently exhibits unexplained degradation using established protocols.
Authors
- Fengtang Yang (ORCID: https://orcid.org/0000-0002-3573-2354)
- Bingrui Sun
- Jicheng Qu
- Ao Li (ORCID: https://orcid.org/0009-0000-6170-8967)
- Ying Zhang (ORCID: https://orcid.org/0000-0001-6808-6240)
- Ping Yu (ORCID: https://orcid.org/0000-0001-8648-9799)
- Hongkuan Deng (ORCID: https://orcid.org/0000-0002-6608-5591)
- Qiuxiang Pang (ORCID: https://orcid.org/0000-0002-0742-9956)
Institutions
- Shandong University of Technology (CN)
Publication Details
- Journal
- BMC Genomics
- Published
- 2025-05-26
- DOI
- https://doi.org/10.1186/s12864-025-11731-6
- Primary Topic
- Marine Ecology and Invasive Species
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Shandong Province