miR-15b-5p affects the innate immune response through PAQR3 in patients with reflux esophagitis

Abstract Objectives Reflux esophagitis (RE) arises from the regurgitation of gastric fluid that contains acids, bile, and other damaging components, significantly impacting on the quality of life. This study aimed to investigate the functions of miR-15b-5p and AdipoQ receptor family member 3 (PAQR3) in reflux esophagitis, as well as to delve into the effect on innate immune response. Methods The study enrolled 54 patients with RE and 33 healthy subjects. qRT-PCR was used to detect the expression level of miR-15b-5p, PAQR3, TLR2, TLR4 and MyD88. The ELISA kits were employed to assess the alterations in the levels of IFN-β, IL-6, and IL-8. Additionally, a dual-luciferase reporter assay was used to verify the binding relationship between miR-15b-5p and PAQR3. Results miR-15b-5p was significantly elevated in RE patients. Cell model tests demonstrated that miR-15b-5p was upregulated in cells treated with acid medium. Inhibition of miR-15b-5p significantly suppressed the expression of innate immunity related genes induced by acid medium. miR-15b-5p targets and regulates PAQR3. In addition, miR-15b-5p activated innate immune responses by blocking PAQR3. Conclusions miR-15b-5p may be involved in the innate immune response of reflux esophagitis by regulating PAQR3.